nested-PCR

基本解释

Detection of Fetal SRY Gene from Maternal Blood by a Nested PCR.
利用套式PCR技术检测母血中胎儿SRY基因的研究
Nested PCR requires two sets of primers which are used to amplify a specific DNA fragment using two separate runs of PCR.
巢式PCR引所需要两套用于放大特定的DNA片段,用两条挤提聚合酶链。
In 47 serum samples which were HCV positive in ELISA,and 33(70.21%) were positive in RT nested PCR,39(82.98%) were positive in RT PCR DDH.
4 7份ELISA检测HCV抗体阳性血清标本 ;RT nested PCR检出阳性标本 33例 (70 .;2 1%25 );RT PCR DDH检出阳性标本 39例 (82
Single cell 5-plex nest PCR for exons 17,19,44,45 and 48 of DMD gene was established.
获取正常男性单个淋巴细胞和无DMD家族史的单个胚胎细胞,行DMD基因外显子17、19、44、45、48的五重巢式PCR,分析扩增成功率、假阳性率和假阴性率。
In addition, there were 2 negative samples in PPD test, while they were positive in nested PCR assay and also in FQ-PCR assay.
在对2份PPD检测阴性而巢式PCR检测阳性的临床样本的检测结果也为阳性。